Malpighian tubules secrete primary urine, most of which is passed into the hindgut where water, ions and essential metabolites are reabsorbed before the fluid is excreted.
2.
HMB-PP is an essential metabolite in most pathogenic bacteria including " Mycobacterium tuberculosis " as well as in malaria parasites, but is absent from the human host.
3.
The mechanisms of toxicity toward target cells exhibited by T6SS substrates are diverse, but tend to involve targeting of highly conserved bacterial structures, including degradation of the cell wall through amidase or glycohydrolase activity, disruption of cell membranes through lipase activity or pore formation, cleavage of DNA, and degradation of the essential metabolite NAD +.